|
Status |
Public on Jan 01, 2009 |
Title |
DF 2 h (Cy3) vs. 0 h (Cy5) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Dermal fibroblasts, IL-1-treated (2 h)
|
Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6
|
Treatment protocol |
Cells were incubated with 20 ng/ml IL-1 for 0, 1, 2, or 4 h.
|
Growth protocol |
Mouse dermal fibroblasts were cultured in DMEM medium containing antibiotics and 10% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Fluorescent cRNA was generated from 5 µg of total RNA using Agilent Two-Color Quick Amp Labeling Kit. The reaction and purification conditions were based on the manufacturer's protocol.
|
|
|
Channel 2 |
Source name |
Dermal fibroblasts, untreated (0 h)
|
Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6
|
Treatment protocol |
Cells were incubated with 20 ng/ml IL-1 for 0, 1, 2, or 4 h.
|
Growth protocol |
Mouse dermal fibroblasts were cultured in DMEM medium containing antibiotics and 10% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
Label |
Cy5
|
Label protocol |
Fluorescent cRNA was generated from 5 µg of total RNA using Agilent Two-Color Quick Amp Labeling Kit. The reaction and purification conditions were based on the manufacturer's protocol.
|
|
|
|
Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially with GE Wash Buffer 1 at room temperature and GE Wash Buffer 2 at 31°C.
|
Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.7.5).
|
Description |
Replicate 2 of 2
|
Data processing |
LOWESS normalized, background-subtracted data obtained from log2 of processed test signal/processed reference signal. Agilent software was used.
|
|
|
Submission date |
Jul 29, 2008 |
Last update date |
Jul 30, 2008 |
Contact name |
Jin Mo Park |
E-mail(s) |
jmpark@cbrc2.mgh.harvard.edu
|
Phone |
617-643-2328
|
Organization name |
Massachusetts General Hospital
|
Department |
Cutaneous Biology Research Center
|
Street address |
149 Thirteenth Street
|
City |
Charlestown |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
|
|
Platform ID |
GPL2872 |
Series (1) |
GSE12285 |
Interleukin-1-induced gene expression in mouse dermal fibroblasts |
|