|
| Status |
Public on Sep 10, 2008 |
| Title |
iDC_DMSO_4hr |
| Sample type |
RNA |
| |
|
| Source name |
iDCs cells, DMSO treated, at 4hour
|
| Organism |
Homo sapiens |
| Characteristics |
iDCs cells, DMSO treated, at 4hour
|
| Treatment protocol |
Human iDCs (10e5 cells/ml) were incubated with 0.1% DMSO (vehicle control), test plant extract or derived phytochemicals in basal medium in 96-well plates for 24 h in a 5% CO2 incubator.
|
| Growth protocol |
Monocyte-derived dendritic cells were generated from peripheral blood mononuclear cells (PBMCs) isolated from healthy volunteers blood samples as described previously [25]. Briefly, CD14+ monocytes were isolated by passing the PBMCs through a magnetic cell separation system (Miltenyi Biotec, Germany). CD14+ cells at 95% purity were cultured for 7 days in AIM-V medium in the presence of GM-CSF (1000 U/ml) and IL-4 (500 U/ml) to obtain immature dendritic cells (iDCs).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
seven micrograms of total RNA from each sample was used to synthesize the first-strand cDNA with T7-Oligo (dT) primer and T7 RNA polymerase by in vitro transcription reaction. The biotinylated cRNA products were then cleaned according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Aliquots of 15 micrograms of total cRNA per sample were hybridized to the Affymetrix gene chip HG-U133A following the manufacturer's instructions
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
effect of 4 hour treatment of DMSO on iDCS
|
| Data processing |
The cell intensities and detection from the nine hybridized oligonucleotide microarrays were derived by use of the Affymetrix Microarray Suite 5 (MAS 5.0) with default settings.
|
| |
|
| Submission date |
Jul 30, 2008 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Ning-Sun Yang |
| E-mail(s) |
nsyang@gate.sinica.edu.tw
|
| Phone |
886-2-2785-1086 ext. 101
|
| Fax |
886-2-2651-5600
|
| URL |
http://abrc.sinica.edu.tw/
|
| Organization name |
Academia Sinica
|
| Department |
Agricultural Biotechnology Research Center
|
| Lab |
Ning-Sun Yang
|
| Street address |
No.128 Sec.2, Academia Rd.
|
| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE12259 |
Human dendritic cells treated with Echinacea purpurea phytocompounds |
|
| Relations |
| Reanalyzed by |
GSE86363 |
