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Sample GSM3094795 Query DataSets for GSM3094795
Status Public on Mar 22, 2021
Title 3_CCCA
Sample type RNA
 
Source name Scalp, affected
Organism Homo sapiens
Characteristics tissue: scalp
ccca status: Affected
patient id: 3
disease state: Central Centrifugal Cicatricial Alopecia
Treatment protocol Samples submerged in 2mL tubes containing RNAlater stabilization reagent, stored at 4°C for 24hr, then at -80◦C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with the QIAGEN RNeasy Fibrous Tissue Mini Kit using manufacturer's protocol.
Label biotin
Label protocol RNA was labeled with the Affymetrix GeneChip WT PLUS Reagent Kit using manufacturer's protocol.
 
Hybridization protocol Hybridized for 17hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
Scan protocol Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
Description Patient 3 Affected
Data processing Affymetrix CEL files were extracted and their data normalized with the Partek GS 6.6 platform. RMA normalization created quantile-normalized log2 transcript-level signal values, which were used in teh subsequent ANOVA analysis. Transcript annotation was subsequently updated.
 
Submission date Apr 12, 2018
Last update date Mar 23, 2021
Contact name Crystal Aguh
E-mail(s) cagi1@jhmi.edu
Organization name Johns Hopkins School of Medicine
Department Dermatology
Street address 1500 Orleans St, CRB II Suite 200
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
 
Platform ID GPL23159
Series (1)
GSE113052 Gene Expression Profile of Central Centrifugal Cicatricial Alopecia

Data table header descriptions
ID_REF
VALUE RMA-normalized, Partek GS v6.6, Log2 gene-level signal value

Data table
ID_REF VALUE
TC0100006437.hg.1 3.44701
TC0100006476.hg.1 6.8267
TC0100006479.hg.1 6.5682
TC0100006480.hg.1 6.43506
TC0100006483.hg.1 4.59747
TC0100006486.hg.1 6.96787
TC0100006490.hg.1 4.48501
TC0100006492.hg.1 5.587
TC0100006494.hg.1 6.92267
TC0100006497.hg.1 5.0158
TC0100006499.hg.1 5.99333
TC0100006501.hg.1 7.10683
TC0100006502.hg.1 4.6374
TC0100006514.hg.1 6.02115
TC0100006516.hg.1 6.91374
TC0100006517.hg.1 4.02019
TC0100006524.hg.1 6.49616
TC0100006540.hg.1 4.52776
TC0100006548.hg.1 4.30825
TC0100006550.hg.1 7.6814

Total number of rows: 21448

Table truncated, full table size 545 Kbytes.




Supplementary file Size Download File type/resource
GSM3094795_CAgu-3NBCCCA-1a-HuClariomS_Clariom_S_Human_.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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