|
| Status |
Public on Apr 13, 2018 |
| Title |
Weight_stable [ID106_065F] |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
weight status: Weight_stable
tissue: White adipose tissue
genotype: female
age: 32
|
| Treatment protocol |
Biopsies from the abdominal subcutaneous adipose tissue depot were obtained by aspiration needle biopsly. Samples were immediately washed in NacCl (9 mg/ml) and thereafter frozen in liquid nitrogen and kept at -70C for subsequent RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy kit (cat no. 217004, Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
The WT Plus Kit was used to generate amplified and biotinylated sense-strand DNA targets from total RNA (Thermo Fisher).
|
| |
|
| Hybridization protocol |
A total of 5.5 µg of fragmented and biotinylated sense-strand DNA target were hybridized to the arrays in a GeneChip Hybridization Oven 645 for 16-18h at 45°C.
|
| Scan protocol |
The arrays were washed and stained in the Gene Chip Fluidics Station 450 followed by scanning in an Affymetrix Gene Chip Scanner 7.
|
| Data processing |
CEL files were analyzed in Affymetrix Expression Console (v. 1.4.1) using the SST-RMA method. Microarray gene expression (log2-values) was analyzed in Qlucore (www.qlucore.com).
|
| |
|
| Submission date |
Apr 12, 2018 |
| Last update date |
Apr 13, 2018 |
| Contact name |
Ingrid Dahlman |
| E-mail(s) |
ingrid.dahlman@ki.se
|
| Organization name |
Karolinska
|
| Street address |
Karolinska Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE113080 |
Altered adipose lipid mobilization predicts long-term weight gain and impaired glucose metabolism |
|
