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Status |
Public on Oct 27, 2008 |
Title |
Bovine Oocyte invivo, biological rep1 |
Sample type |
RNA |
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Source name |
Bovine Invivo cultured Oocytes
|
Organism |
Bos taurus |
Characteristics |
In vivo derived: Oocyte
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Poly(A)+ RNA was isolated with a Dynabeads mRNA Direct Kit (Invitrogen) according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
biotin-labeled cRNA was prepared in an in vitro transcription reaction using the GeneChip IVT Labeling Kit (Affymetrix).
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Hybridization protocol |
Ten μg of fragmented cRNA was used for hybridization on the GenChip Bovine Genome Array (Affymetrix) overnight at 45°C according to the manufacture’s instructions.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
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Description |
Gene expression data from Invivo cultured Oocyte
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Data processing |
The Bioconductor open source library was used for initial processing of the raw files. CEL files were preprocessed using the GCRMA algorithm of the GCRMA package [v2.0.0] and detection calls were obtained using the mas5calls function of the Affymetrix platform.
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Submission date |
Aug 04, 2008 |
Last update date |
Aug 04, 2008 |
Contact name |
Smita Sudheer |
E-mail(s) |
sudheer@molgen.mpg.de, adjaye@molgen.mpg.de, kues@tzv.fal.de, niemann@tzv.fal.de
|
Organization name |
Max Planck Institute for Molecular Genetics
|
Street address |
Ihnestr. 63-73
|
City |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE12327 |
Expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation in vivo |
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