|
| Status |
Public on Oct 27, 2008 |
| Title |
Bovine Blastocyst, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Bovine preimplantation embryo Invivo derived Blastocyst
|
| Organism |
Bos taurus |
| Characteristics |
In vivo derived: Blastocyst
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Poly(A)+ RNA was isolated with a Dynabeads mRNA Direct Kit (Invitrogen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
biotin-labeled cRNA was prepared in an in vitro transcription reaction using the GeneChip IVT Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Ten μg of fragmented cRNA was used for hybridization on the GenChip Bovine Genome Array (Affymetrix) overnight at 45°C according to the manufacture’s instructions.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
Gene expression data from preimplantation embryos Blastocyst
|
| Data processing |
The Bioconductor open source library was used for initial processing of the raw files. CEL files were preprocessed using the GCRMA algorithm of the GCRMA package [v2.0.0] and detection calls were obtained using the mas5calls function of the Affymetrix platform.
|
| |
|
| Submission date |
Aug 04, 2008 |
| Last update date |
Aug 04, 2008 |
| Contact name |
Smita Sudheer |
| E-mail(s) |
sudheer@molgen.mpg.de, adjaye@molgen.mpg.de, kues@tzv.fal.de, niemann@tzv.fal.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Street address |
Ihnestr. 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE12327 |
Expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation in vivo |
|
