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Sample GSM30973 Query DataSets for GSM30973
Status Public on Sep 30, 2004
Title F1#68 U34B
Sample type RNA
 
Source name Heart, Left Ventricle
Organism Rattus norvegicus
Extracted molecule total RNA
 
Description RNA was extracted from the left ventricle of the heart, taken from a single 15 week old inbred rat that was maintained under specific pathogen free conditions. Target cRNA was prepared using the Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), purified on a column (Rneasy mini kit, Qiagen), and hybridized to commercially available Affymetrix rat GeneChips arrays according to protocols recommended by the manufacturers. Data was analyzed using the GeneChip Expression Analysis Software (Affymetrix).
Keywords = quantitative real-time RT-PCR
Keywords = insulin receptor substrate 2 (Irs2)
Keywords = phosphatidylinositol 3-kinase
Keywords = regulatory subunit 1
Keywords = (Pik3r1)
Keywords = endurance running
Keywords = treadmill running
 
Submission date Sep 24, 2004
Last update date May 27, 2005
Contact name Justin A. Ways
E-mail(s) jways@mco.edu
Phone 419-383-4150
Organization name Medical College of Ohio
Department Physiology and Cardiovascular Genomics
Street address 3035 Arlington Ave.
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platform ID GPL86
Series (1)
GSE1795 Expression Profiling of DA, COP, and F1(COPxDA) rat left ventricles

Data table header descriptions
ID_REF
VALUE normalized signal representing quantity of transcript
ABS_CALL absolute detection call where P=present, A=absent, and M=marginal
DETECTION P-VALUE measure of the significance of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 8.4 A 0.824672
AFFX-MurIL10_at 6.4 A 0.897835
AFFX-MurIL4_at 2 A 0.957038
AFFX-MurFAS_at 6.2 A 0.804734
AFFX-BioB-5_at 212.3 P 0.036569
AFFX-BioB-M_at 314.7 P 0.00039
AFFX-BioB-3_at 177.8 P 0.011384
AFFX-BioC-5_at 761.6 P 0.000297
AFFX-BioC-3_at 499.7 P 0.000754
AFFX-BioDn-5_at 718.9 P 0.00141
AFFX-BioDn-3_at 4208 P 0.000147
AFFX-CreX-5_at 9056 P 0.000044
AFFX-CreX-3_at 10089.9 P 0.000044
AFFX-BioB-5_st 73.9 A 0.216524
AFFX-BioB-M_st 57.9 A 0.48511
AFFX-BioB-3_st 17.4 A 0.883887
AFFX-BioC-5_st 14.7 A 0.921998
AFFX-BioC-3_st 7.4 A 0.860518
AFFX-BioDn-5_st 87.4 A 0.239063
AFFX-BioDn-3_st 95.1 A 0.123572

Total number of rows: 8791

Table truncated, full table size 266 Kbytes.




Supplementary data files not provided

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