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Sample GSM30974 Query DataSets for GSM30974
Status Public on Sep 30, 2004
Title F1#69 U34B
Sample type RNA
 
Source name Heart, Left Ventricle
Organism Rattus norvegicus
Extracted molecule total RNA
 
Description RNA was extracted from the left ventricle of the heart, taken from a single 15 week old inbred rat that was maintained under specific pathogen free conditions. Target cRNA was prepared using the Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), purified on a column (Rneasy mini kit, Qiagen), and hybridized to commercially available Affymetrix rat GeneChips arrays according to protocols recommended by the manufacturers. Data was analyzed using the GeneChip Expression Analysis Software (Affymetrix).
Keywords = quantitative real-time RT-PCR
Keywords = insulin receptor substrate 2 (Irs2)
Keywords = phosphatidylinositol 3-kinase
Keywords = regulatory subunit 1
Keywords = (Pik3r1)
Keywords = endurance running
Keywords = treadmill running
 
Submission date Sep 24, 2004
Last update date May 27, 2005
Contact name Justin A. Ways
E-mail(s) jways@mco.edu
Phone 419-383-4150
Organization name Medical College of Ohio
Department Physiology and Cardiovascular Genomics
Street address 3035 Arlington Ave.
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platform ID GPL86
Series (1)
GSE1795 Expression Profiling of DA, COP, and F1(COPxDA) rat left ventricles

Data table header descriptions
ID_REF
VALUE normalized signal representing quantity of transcript
ABS_CALL absolute detection call where P=present, A=absent, and M=marginal
DETECTION P-VALUE measure of the significance of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 5.7 A 0.876428
AFFX-MurIL10_at 11.5 A 0.891021
AFFX-MurIL4_at 1.9 A 0.904333
AFFX-MurFAS_at 5.8 A 0.749204
AFFX-BioB-5_at 241.2 P 0.021902
AFFX-BioB-M_at 336.8 P 0.000857
AFFX-BioB-3_at 201.9 P 0.009337
AFFX-BioC-5_at 673 P 0.000169
AFFX-BioC-3_at 426.3 P 0.000662
AFFX-BioDn-5_at 666.6 P 0.00141
AFFX-BioDn-3_at 3932.2 P 0.000127
AFFX-CreX-5_at 8262.9 P 0.000044
AFFX-CreX-3_at 9960.6 P 0.000044
AFFX-BioB-5_st 53.7 A 0.313723
AFFX-BioB-M_st 48.8 A 0.617401
AFFX-BioB-3_st 16.4 A 0.916408
AFFX-BioC-5_st 11 A 0.921998
AFFX-BioC-3_st 6.3 A 0.814869
AFFX-BioDn-5_st 37.2 A 0.287743
AFFX-BioDn-3_st 82.7 A 0.131361

Total number of rows: 8791

Table truncated, full table size 266 Kbytes.




Supplementary data files not provided

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