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Sample GSM30977 Query DataSets for GSM30977
Status Public on Sep 30, 2004
Title DA#57 U34B
Sample type RNA
 
Source name Heart, Left Ventricle
Organism Rattus norvegicus
Extracted molecule total RNA
 
Description RNA was extracted from the left ventricle of the heart, taken from a single 15 week old inbred rat that was maintained under specific pathogen free conditions. Target cRNA was prepared using the Enzo Bioarray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), purified on a column (Rneasy mini kit, Qiagen), and hybridized to commercially available Affymetrix rat GeneChips arrays according to protocols recommended by the manufacturers. Data was analyzed using the GeneChip Expression Analysis Software (Affymetrix).
Keywords = quantitative real-time RT-PCR
Keywords = insulin receptor substrate 2 (Irs2)
Keywords = phosphatidylinositol 3-kinase
Keywords = regulatory subunit 1
Keywords = (Pik3r1)
Keywords = endurance running
Keywords = treadmill running
 
Submission date Sep 24, 2004
Last update date May 27, 2005
Contact name Justin A. Ways
E-mail(s) jways@mco.edu
Phone 419-383-4150
Organization name Medical College of Ohio
Department Physiology and Cardiovascular Genomics
Street address 3035 Arlington Ave.
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platform ID GPL86
Series (1)
GSE1795 Expression Profiling of DA, COP, and F1(COPxDA) rat left ventricles

Data table header descriptions
ID_REF
VALUE normalized signal representing quantity of transcript
ABS_CALL absolute detection call where P=present, A=absent, and M=marginal
DETECTION P-VALUE measure of the significance of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 6.9 A 0.876428
AFFX-MurIL10_at 7.9 A 0.852061
AFFX-MurIL4_at 1.5 A 0.941556
AFFX-MurFAS_at 3.4 A 0.868639
AFFX-BioB-5_at 157 A 0.102165
AFFX-BioB-M_at 270.6 P 0.004484
AFFX-BioB-3_at 136.6 P 0.033677
AFFX-BioC-5_at 768.8 P 0.000446
AFFX-BioC-3_at 422.1 P 0.001248
AFFX-BioDn-5_at 439.9 P 0.001248
AFFX-BioDn-3_at 4002.2 P 0.000127
AFFX-CreX-5_at 6946.3 P 0.000044
AFFX-CreX-3_at 9152.3 P 0.000044
AFFX-BioB-5_st 53.6 A 0.368438
AFFX-BioB-M_st 86.7 A 0.48511
AFFX-BioB-3_st 15.4 A 0.921998
AFFX-BioC-5_st 12.8 A 0.941556
AFFX-BioC-3_st 17.1 A 0.749204
AFFX-BioDn-5_st 120.2 A 0.156732
AFFX-BioDn-3_st 118 A 0.083592

Total number of rows: 8791

Table truncated, full table size 266 Kbytes.




Supplementary data files not provided

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