|
| Status |
Public on Aug 21, 2019 |
| Title |
CIP02 |
| Sample type |
RNA |
| |
|
| Source name |
primary GBM cells from peripheral region of GBM tumor
|
| Organism |
Homo sapiens |
| Characteristics |
sample region: Periphery region
cell type: Primary Glioblastoma (GBM) Cells
sample code: HuTuP109
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cryopreserved GBM tissues of different tumor regions using Trizol
|
| Label |
biotin
|
| Label protocol |
Labeled and Fragmented ss-cDNA were prepared according to the standard GeneChip WT Plus protocol starting from 10-50 ng of Total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation and labelling,5.5 μg ss-cDNA were hybridized for 16 hr at 45C on GeneChip Human Genome Clariom S Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from primary GBM cells from peripheral region of GBM tumor
|
| Data processing |
The data were normalized using R software [www.r-project.org] with BioConductor package [www.bioconductor.org] using RMA
|
| |
|
| Submission date |
Apr 23, 2018 |
| Last update date |
Aug 21, 2019 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE113512 |
A HIF-1α/Wnt signaling-dependent control of gene transcription regulates neuronal differentiation of glioblastoma stem cells |
|
