|
Status |
Public on Aug 21, 2019 |
Title |
CIP09 |
Sample type |
RNA |
|
|
Source name |
primary GBM cells from internal region of GBM tumor
|
Organism |
Homo sapiens |
Characteristics |
sample region: Internal region cell type: Primary Glioblastoma (GBM) Cells sample code: HuTuP119
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from cryopreserved GBM tissues of different tumor regions using Trizol
|
Label |
biotin
|
Label protocol |
Labeled and Fragmented ss-cDNA were prepared according to the standard GeneChip WT Plus protocol starting from 10-50 ng of Total RNA
|
|
|
Hybridization protocol |
Following fragmentation and labelling,5.5 μg ss-cDNA were hybridized for 16 hr at 45C on GeneChip Human Genome Clariom S Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
Description |
Gene expression data from primary GBM cells from internal region of GBM tumor
|
Data processing |
The data were normalized using R software [www.r-project.org] with BioConductor package [www.bioconductor.org] using RMA
|
|
|
Submission date |
Apr 23, 2018 |
Last update date |
Aug 21, 2019 |
Contact name |
silvia bresolin |
E-mail(s) |
silvia.bresolin@unipd.it
|
Phone |
+390498215487
|
Organization name |
università di padova
|
Department |
department of women's and children's health
|
Lab |
SSD ematologia-clinica sperimentale
|
Street address |
via giustiniani 3
|
City |
padova |
State/province |
padova |
ZIP/Postal code |
35128 |
Country |
Italy |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE113512 |
A HIF-1α/Wnt signaling-dependent control of gene transcription regulates neuronal differentiation of glioblastoma stem cells |
|