|
| Status |
Public on Apr 26, 2018 |
| Title |
HBL-1 treated by EDA replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
HBL-1 treated by EDA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HBL-1
cell type: B Lymphoma cell line
treated with: EDA (1µg/ml) for 6hrs
|
| Treatment protocol |
BJAB and HBL-1 cell lines were stimulated for 6 hours with recombinant purified APRIL (1µg/ml) or with EDA (1µg/ml) as control.
|
| Growth protocol |
BJAB and HBL-1 cell lines were cultured in RPMI1640 medium and 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were purified from cells with the RNeasy Qiagen purification kit following manufacturer's instructions including an on-column DNase I treatment step to remove any residual contaminant DNA.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
BH1
RNA expression data from HBL-1 treated by EDA
|
| Data processing |
Data obtained for the whole set of samples were normalized by RMA.
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Apr 25, 2018 |
| Last update date |
Apr 26, 2018 |
| Contact name |
Jerome Moreaux |
| E-mail(s) |
jerome.moreaux@igh.cnrs.fr
|
| Organization name |
CHRU Montpellier
|
| Department |
Biological Hematology
|
| Lab |
Laboratory for Monitoring Innovative Therapies
|
| Street address |
80 Av. Augustin Fliche
|
| City |
Montpellier |
| ZIP/Postal code |
34295 |
| Country |
France |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE113643 |
Coreceptor delivery of paracrine APRIL to Golgic BCMA lowers immunogenicity of B-cell lymphoma |
|
