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Sample GSM311461 Query DataSets for GSM311461
Status Public on Feb 28, 2009
Title AGTR2 knockout replicate 4
Sample type RNA
 
Channel 1
Source name whole brain E15 Knockout
Organism Mus musculus
Characteristics C57BL/6 x 129;male;E15
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen slices of whole brain using the Qiagen Rneasy kit according to manufacturer instructions followed by Dnase with the Ambion Turbo Dnase-free kit
Label Cy5
Label protocol Agilent low RNA input linear amplification kit with Cy3 or Cy5-CTP according to maunfacturer instructions
 
Channel 2
Source name whole brain E15 control replicate one
Organism Mus musculus
Characteristics C57BL/6 x 129;male;E15
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen slices of whole brain using the Qiagen Rneasy kit according to manufacturer instructions followed by Dnase with the Ambion Turbo Dnase-free kit
Label Cy3
Label protocol Agilent low RNA input linear amplification kit with Cy3 or Cy5-CTP according to maunfacturer instructions
 
 
Hybridization protocol Agilent hybridization protocol for 17 hours at 65 degrees Celcius
Scan protocol Agilent scanner G2505B, 10 micron resolution extracted with Agilent Feature Extraction v 8.0 software
Description none
Data processing LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Aug 11, 2008
Last update date Dec 31, 2008
Contact name traci pawlowski
E-mail(s) tpawlowski@tgen.org
Phone 602-343-8574
Organization name Greenwood Genetic Ctr
Department research
Lab Srivastava
Street address 125 Gregor Mendel Circle
City Greenwood
State/province SC
ZIP/Postal code 29646
Country USA
 
Platform ID GPL2872
Series (1)
GSE12412 Embryonic day 15 and Postnatal day 1 whole mouse brain AGTR2 knockout versus control

Data table header descriptions
ID_REF
VALUE normalized log2 ratio representing test/reference

Data table
ID_REF VALUE
1 -1.20E+00
2 0.00E+00
3 -1.06E-01
4 -5.99E-02
5 -3.09E-01
6 -3.50E-02
7 -1.63E+00
8 -3.14E-01
9 -5.29E-02
10 5.97E-02
11 -2.00E-02
12 -8.35E-02
13 -3.07E-02
14 -1.68E+00
15 0.00E+00
16 7.04E-03
17 -7.48E-02
18 3.39E-02
19 8.34E-02
20 -1.66E-02

Total number of rows: 43790

Table truncated, full table size 650 Kbytes.




Supplementary file Size Download File type/resource
GSM311461.txt.gz 11.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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