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Sample GSM311476 Query DataSets for GSM311476
Status Public on Dec 15, 2008
Title N2-controlRNAi-fasting-biological rep1
Sample type RNA
 
Source name Wild Type N2, N2-controlRNAi-fasting-biological rep1
Organism Caenorhabditis elegans
Characteristics Age: Day 4 of adulthood.
Treatment protocol Worms grown on RNAi plates (control RNAi or Cel-Rheb RNAi or Cel-TOR RNAi) were fed ad libitum or fasted for 48 h.
Growth protocol All worms are grown at 20 degrees C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated and purified by following the manual of Sepasol(R)-RNA Ⅰ Super (nakalai tesque) and RNeasy mini kit (Qiagen), respectively.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45 degrees C on C. elegans Genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner.
Description Gene expression data from wild type worms at day 4 of adulthood. Worms were grown on control RNAi plates and fasted for 48 h.
Data processing The data were analyzed with GCRMA algorithm
 
Submission date Aug 11, 2008
Last update date Dec 13, 2008
Contact name Eisuke Nishida
E-mail(s) nishida@lif.kyoto-u.ac.jp
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
 
Platform ID GPL200
Series (1)
GSE9682 Dietary restriction in Caenorhabditis elegans

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity (GeneSpring GX 7.3.1).

Data table
ID_REF VALUE
171720_x_at 276.4113159
171721_x_at 1893.11438
171722_x_at 957.4368286
171723_x_at 37207.91406
171724_x_at 2392.259277
171725_x_at 161.696167
171726_x_at 98.72249603
171727_x_at 16.4390564
171728_x_at 36.84876251
171729_x_at 1024.444946
171730_x_at 10.23757267
171731_x_at 1022.722473
171732_x_at 523.8846436
171733_x_at 153.38797
171734_x_at 11774.86035
171735_x_at 136.4537354
171736_x_at 967.0220947
171737_x_at 2695.668945
171738_x_at 498.0160522
171739_x_at 123.8317719

Total number of rows: 22625

Table truncated, full table size 497 Kbytes.




Supplementary file Size Download File type/resource
GSM311476.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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