|
| Status |
Public on Dec 15, 2008 |
| Title |
N2-Cel-RhebRNAi-fasting-biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild Type N2, N2-Cel-RhebRNAi-fasting-biological rep2
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
Age: Day 4 of adulthood.
|
| Treatment protocol |
Worms grown on RNAi plates (control RNAi or Cel-Rheb RNAi or Cel-TOR RNAi) were fed ad libitum or fasted for 48 h.
|
| Growth protocol |
All worms are grown at 20 degrees C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated and purified by following the manual of Sepasol(R)-RNA Ⅰ Super (nakalai tesque) and RNeasy mini kit (Qiagen), respectively.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45 degrees C on C. elegans Genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from wild type worms at day 4 of adulthood. Worms were grown on Cel-Rheb RNAi plates and fasted for 48 h.
|
| Data processing |
The data were analyzed with GCRMA algorithm
|
| |
|
| Submission date |
Aug 11, 2008 |
| Last update date |
Dec 13, 2008 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE9682 |
Dietary restriction in Caenorhabditis elegans |
|
