|
| Status |
Public on Jul 25, 2009 |
| Title |
Left atria dnPI3K 3241 |
| Sample type |
RNA |
| |
|
| Source name |
RNA from mouse heart atria, dnPI3K
|
| Organism |
Mus musculus |
| Characteristics |
male C57BL/6/FVB/N
|
| Treatment protocol |
Transgenics models dnPI3k, Mst1 and dnPI3K-Mst1 were made accordingly.
|
| Growth protocol |
The mice were on a standard chow diet, and were kept in temperature controlled cages (21 - 23 degree celcius) and maintained a 12 hour light/dark cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heart was homogenized individually using motar and pestle in liquid nitrogen. Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Fragmented and biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA for Affymetrix Mouse Gene 1.0 ST arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were processed through second-cycle single stranded cDNA, fragmented and labelled cDNA were then hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Gene arrays were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from heart atria
|
| Data processing |
Gene array data is processd by Expression Console software in which mean probeset signals for all probesets are analysed. A receiver operating curve (ROC) is generated by thrseholding the computed value for the probesets. PLIER signals that pass this QC control is used for subsequent analysis.
|
| |
|
| Submission date |
Aug 12, 2008 |
| Last update date |
Jul 24, 2009 |
| Contact name |
Ruby CY Lin |
| E-mail(s) |
rubyl@unsw.edu.au, ruby.lin@sydney.edu.au
|
| Organization name |
The Westmead Institute for Medical Research
|
| Street address |
176 Hawkesbury Road, Westmead
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE12420 |
Gene profiling of heart atria in PI3K and Mst1 mouse models |
|
