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Sample GSM312688 Query DataSets for GSM312688
Status Public on Aug 31, 2008
Title 224 Day 1 siMut10 transfected biological rep2
Sample type RNA
 
Source name cultured human melanoma cancer cell 224
Organism Homo sapiens
Characteristics cell line
Treatment protocol NRAS-MUT10 (siMut10) transfected cells from Day 1
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured melanoma cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Aug 14, 2008
Last update date Sep 01, 2016
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE12445 Expression data from melanoma cell lines 224 and BL transfected with siRNA-NRASQ61R
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 4265.4 P 0.000562
1053_at 2600.1 P 0.001354
117_at 159.3 A 0.418069
121_at 997.3 P 0.013092
1255_g_at 44.7 A 0.951033
1294_at 363.9 A 0.32083
1316_at 282.2 A 0.250724
1320_at 280.9 A 0.267463
1405_i_at 169.9 A 0.681158
1431_at 215.5 A 0.479377
1438_at 59.6 A 0.824051
1487_at 1180.6 P 0.039365
1494_f_at 329 A 0.267463
1598_g_at 960.1 A 0.189687
160020_at 1518.4 P 0.001602
1729_at 965.4 P 0.006532
177_at 686.1 P 0.005643
1773_at 1623.9 P 0.001892
179_at 299.1 A 0.621835
1861_at 604.7 A 0.098054

Total number of rows: 22283

Table truncated, full table size 602 Kbytes.




Supplementary file Size Download File type/resource
GSM312688.cel.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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