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Sample GSM312708 Query DataSets for GSM312708
Status Public on Aug 31, 2008
Title BL Day 0 Untransfected biological rep1
Sample type RNA
 
Source name cultured human melanoma cancer cell BL
Organism Homo sapiens
Characteristics cell line
Treatment protocol Untransfected cells from Day 0
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured melanoma cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Aug 14, 2008
Last update date Sep 01, 2016
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE12445 Expression data from melanoma cell lines 224 and BL transfected with siRNA-NRASQ61R
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 2212.8 P 0.000468
1053_at 4042.4 P 0.000322
117_at 172.3 A 0.378184
121_at 901.7 P 0.02786
1255_g_at 22.6 A 0.872355
1294_at 275 A 0.098054
1316_at 168.2 A 0.250724
1320_at 83.5 A 0.458816
1405_i_at 72.3 A 0.462489
1431_at 116.9 A 0.602023
1438_at 51.1 A 0.697453
1487_at 1081 P 0.013092
1494_f_at 188.3 A 0.267428
1598_g_at 2110.3 P 0.000805
160020_at 929.4 P 0.006532
1729_at 349.7 M 0.060388
177_at 143 A 0.107301
1773_at 641.8 P 0.014937
179_at 393 A 0.458816
1861_at 907.6 P 0.000562

Total number of rows: 22283

Table truncated, full table size 601 Kbytes.




Supplementary file Size Download File type/resource
GSM312708.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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