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Sample GSM312734 Query DataSets for GSM312734
Status Public on Aug 31, 2008
Title BL Day 2 siSC transfected biological rep4
Sample type RNA
 
Source name cultured human melanoma cancer cell BL
Organism Homo sapiens
Characteristics cell line
Treatment protocol scrambled siRNA (siSC) transfected cells from Day 2
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured melanoma cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Aug 14, 2008
Last update date Sep 01, 2016
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE12445 Expression data from melanoma cell lines 224 and BL transfected with siRNA-NRASQ61R
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 3680.1 P 0.001602
1053_at 2671.6 P 0.000562
117_at 199.8 A 0.175989
121_at 1316.6 P 0.013092
1255_g_at 71.5 A 0.660442
1294_at 303.9 A 0.189687
1316_at 64.8 A 0.52062
1320_at 123.7 A 0.52062
1405_i_at 24.4 A 0.877253
1431_at 22.8 A 0.960635
1438_at 296.8 A 0.284747
1487_at 1803 P 0.014937
1494_f_at 411.5 A 0.162935
1598_g_at 2722.1 P 0.008689
160020_at 894.2 P 0.043968
1729_at 184.3 A 0.204022
177_at 349.6 A 0.081337
1773_at 585.3 P 0.048995
179_at 1229.4 A 0.204022
1861_at 666 M 0.05447

Total number of rows: 22283

Table truncated, full table size 601 Kbytes.




Supplementary file Size Download File type/resource
GSM312734.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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