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Sample GSM3133389 Query DataSets for GSM3133389
Status Public on May 07, 2021
Title CON rep4
Sample type SRA
 
Source name Lung
Organism Mus musculus
Characteristics strain: ICR
age: 7-9 weeks
genotype/variation:
treatment: control
Treatment protocol After intraperitoneal anesthesia with ketamine at 100 mg/kg, mice were fixed in a supine position, then endotracheal intubation was performed, and the tube was connected to a rodent ventilator. Ventilation was performed at tidal volume of 30 ml/kg and respiratory rate of 70 breaths/min for 4 h. Mice in sham group underwent intubation but breathed freely. At the end of experiment, animals were sacrificed by anesthetic overdose, and the lung tissues were harvested for further detections.
Growth protocol Mice were housed in a temperature controlled room (22±2°C) with 12 h dark /12 h light cycle and given ad libitum access to food and water.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
Ion Proton Sequencing Protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
 
Description All_Counts.txt
Data processing Reads filtering under criteria removing reads with 20% of the base quality lower than 13
Mapsplice v2.2.0 mapping to reference genome
count counting
Genome_build: GRCm38(mm10)
Supplementary_files_format_and_content: TXT file contain counts for each sample
 
Submission date May 07, 2018
Last update date May 07, 2021
Contact name Bo Xu
E-mail(s) xubo@xinhuamed.com.cn
Phone +8613761146277
Organization name Xinhua Hospital, Shanghai Jiaotong University, School of Medicine
Department Department of Anesthesiology and SICU
Street address 1665 Kongjiang Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200092
Country China
 
Platform ID GPL18635
Series (1)
GSE114132 RNA-sequencing analysis of aberrantly expressed long non-coding RNAs and mRNAs in a mouse model of ventilator-induced lung injury
Relations
BioSample SAMN09083355
SRA SRX4048945

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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