|
Status |
Public on May 07, 2021 |
Title |
CON rep5 |
Sample type |
SRA |
|
|
Source name |
Lung
|
Organism |
Mus musculus |
Characteristics |
strain: ICR age: 7-9 weeks genotype/variation: treatment: control
|
Treatment protocol |
After intraperitoneal anesthesia with ketamine at 100 mg/kg, mice were fixed in a supine position, then endotracheal intubation was performed, and the tube was connected to a rodent ventilator. Ventilation was performed at tidal volume of 30 ml/kg and respiratory rate of 70 breaths/min for 4 h. Mice in sham group underwent intubation but breathed freely. At the end of experiment, animals were sacrificed by anesthetic overdose, and the lung tissues were harvested for further detections.
|
Growth protocol |
Mice were housed in a temperature controlled room (22±2°C) with 12 h dark /12 h light cycle and given ad libitum access to food and water.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. Ion Proton Sequencing Protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Ion Torrent Proton |
|
|
Description |
All_Counts.txt
|
Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13 Mapsplice v2.2.0 mapping to reference genome count counting Genome_build: GRCm38(mm10) Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
May 07, 2018 |
Last update date |
May 07, 2021 |
Contact name |
Bo Xu |
E-mail(s) |
xubo@xinhuamed.com.cn
|
Phone |
+8613761146277
|
Organization name |
Xinhua Hospital, Shanghai Jiaotong University, School of Medicine
|
Department |
Department of Anesthesiology and SICU
|
Street address |
1665 Kongjiang Road
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200092 |
Country |
China |
|
|
Platform ID |
GPL18635 |
Series (1) |
GSE114132 |
RNA-sequencing analysis of aberrantly expressed long non-coding RNAs and mRNAs in a mouse model of ventilator-induced lung injury |
|
Relations |
BioSample |
SAMN09083354 |
SRA |
SRX4048946 |