|
| Status |
Public on Aug 19, 2008 |
| Title |
E14_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells wild type
|
| Organism |
Mus musculus |
| Characteristics |
Strain: E14 mouse ES cells
|
| Growth protocol |
For microarray transcriptome profiling of Sall4- and control shRNA treated cells, cells were transfected with appropriate plasmids and cultured under puromycin selection for 3, 4 or 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and column-purified with RNeasy kits (Qiagen).
|
| Label |
biotin
|
| Label protocol |
According to Illumina Protocol
|
| |
|
| Hybridization protocol |
According to Illumina Protocol
|
| Scan protocol |
Illumina BeadScan
|
| Description |
no additional information
|
| Data processing |
Data were normalized with cross-correlation method; differential expression was based on the mean of the log2 fold changes (see GSE12482 supplementary files).
|
| |
|
| Submission date |
Aug 19, 2008 |
| Last update date |
Aug 19, 2008 |
| Contact name |
Bing Lim |
| E-mail(s) |
limb1@gis.a-star.edu.sg
|
| Phone |
(65)64788153
|
| Organization name |
GIS
|
| Department |
Stem Cell and Developmental Biology
|
| Street address |
60 Biopolis Street
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138671 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE12482 |
Sall4 regulates distinct transcription circuitries in different blastocyst-derived stem cell lineages |
|
