|
Status |
Public on Aug 19, 2008 |
Title |
Xen Scrb KD_rep2 |
Sample type |
RNA |
|
|
Source name |
Xen KD control
|
Organism |
Mus musculus |
Characteristics |
Strain: Xen cells from E4.5 blastocysts
|
Growth protocol |
For microarray transcriptome profiling of Sall4- and control shRNA treated cells, cells were transfected with appropriate plasmids and cultured under puromycin selection for 3, 4 or 5 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and column-purified with RNeasy kits (Qiagen).
|
Label |
biotin
|
Label protocol |
According to Illumina Protocol
|
|
|
Hybridization protocol |
According to Illumina Protocol
|
Scan protocol |
Illumina BeadScan
|
Description |
no additional information
|
Data processing |
Data were normalized with cross-correlation method; differential expression was based on the mean of the log2 fold changes (see GSE12482 supplementary files).
|
|
|
Submission date |
Aug 19, 2008 |
Last update date |
Aug 19, 2008 |
Contact name |
Bing Lim |
E-mail(s) |
limb1@gis.a-star.edu.sg
|
Phone |
(65)64788153
|
Organization name |
GIS
|
Department |
Stem Cell and Developmental Biology
|
Street address |
60 Biopolis Street
|
City |
Singapore |
State/province |
Singapore |
ZIP/Postal code |
138671 |
Country |
Singapore |
|
|
Platform ID |
GPL6103 |
Series (1) |
GSE12482 |
Sall4 regulates distinct transcription circuitries in different blastocyst-derived stem cell lineages |
|