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Sample GSM313613 Query DataSets for GSM313613
Status Public on Aug 19, 2008
Title Xen Scrb KD_rep3
Sample type RNA
 
Source name Xen KD control
Organism Mus musculus
Characteristics Strain: Xen cells from E4.5 blastocysts
Growth protocol For microarray transcriptome profiling of Sall4- and control shRNA treated cells, cells were transfected with appropriate plasmids and cultured under puromycin selection for 3, 4 or 5 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and column-purified with RNeasy kits (Qiagen).
Label biotin
Label protocol According to Illumina Protocol
 
Hybridization protocol According to Illumina Protocol
Scan protocol Illumina BeadScan
Description no additional information
Data processing Data were normalized with cross-correlation method; differential expression was based on the mean of the log2 fold changes (see GSE12482 supplementary files).
 
Submission date Aug 19, 2008
Last update date Aug 19, 2008
Contact name Bing Lim
E-mail(s) limb1@gis.a-star.edu.sg
Phone (65)64788153
Organization name GIS
Department Stem Cell and Developmental Biology
Street address 60 Biopolis Street
City Singapore
State/province Singapore
ZIP/Postal code 138671
Country Singapore
 
Platform ID GPL6103
Series (1)
GSE12482 Sall4 regulates distinct transcription circuitries in different blastocyst-derived stem cell lineages

Data table header descriptions
ID_REF
VALUE cross-correlation normalized signal intensity

Data table
ID_REF VALUE
ILMN_2638923 13041.805
ILMN_3162403 50
ILMN_3163582 51.412555
ILMN_3161639 2951.9472
ILMN_3162754 144.64805
ILMN_3162129 51.412555
ILMN_3160253 51.412555
ILMN_3162224 2892.4173
ILMN_3160610 51.412555
ILMN_3161596 51.412555
ILMN_3162495 53.636515
ILMN_3162089 3712.6746
ILMN_3160881 1520.4941
ILMN_2588050 49244.917
ILMN_3160348 66.764083
ILMN_3162388 897.98314
ILMN_3162771 807.31308
ILMN_3162316 51.412555
ILMN_3162695 51.412555
ILMN_3160817 6731.4859

Total number of rows: 24607

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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