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Sample GSM313619 Query DataSets for GSM313619
Status Public on Aug 19, 2008
Title E14 Scrb KD_rep3
Sample type RNA
 
Source name E14 KD control
Organism Mus musculus
Characteristics Strain: E14 mouse ES cells
Growth protocol For microarray transcriptome profiling of Sall4- and control shRNA treated cells, cells were transfected with appropriate plasmids and cultured under puromycin selection for 3, 4 or 5 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and column-purified with RNeasy kits (Qiagen).
Label biotin
Label protocol According to Illumina Protocol
 
Hybridization protocol According to Illumina Protocol
Scan protocol Illumina BeadScan
Description no additional information
Data processing Data were normalized with cross-correlation method; differential expression was based on the mean of the log2 fold changes (see GSE12482 supplementary files).
 
Submission date Aug 19, 2008
Last update date Aug 19, 2008
Contact name Bing Lim
E-mail(s) limb1@gis.a-star.edu.sg
Phone (65)64788153
Organization name GIS
Department Stem Cell and Developmental Biology
Street address 60 Biopolis Street
City Singapore
State/province Singapore
ZIP/Postal code 138671
Country Singapore
 
Platform ID GPL6103
Series (1)
GSE12482 Sall4 regulates distinct transcription circuitries in different blastocyst-derived stem cell lineages

Data table header descriptions
ID_REF
VALUE cross-correlation normalized signal intensity

Data table
ID_REF VALUE
ILMN_2638923 2492.2857
ILMN_3162403 61.186179
ILMN_3163582 61.186179
ILMN_3161639 1639.289
ILMN_3162754 259.02256
ILMN_3162129 61.186179
ILMN_3160253 95.859238
ILMN_3162224 3264.4502
ILMN_3160610 61.186179
ILMN_3161596 63.221816
ILMN_3162495 60.545815
ILMN_3162089 1224.9495
ILMN_3160881 1726.2136
ILMN_2588050 26232.363
ILMN_3160348 386.69445
ILMN_3162388 671.24223
ILMN_3162771 1163.3461
ILMN_3162316 79.352756
ILMN_3162695 61.186179
ILMN_3160817 2018.8864

Total number of rows: 24607

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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