|
| Status |
Public on Nov 13, 2019 |
| Title |
Control replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue of mice (16h fasting)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
background strain: C57BL/6
age: 4 months
Sex: male
treatment: metaformin control
genoype: TGB_empty-vector WT
|
| Treatment protocol |
10 weeks double floxed homozygous AMPK alpha 1 and alpha2 mice were injected with AAV8-TBG-empty vector or AAV8-TBG-Cre (1x10^11 GC/mouse) through jugular vein, and then, treated with metformin (50 mg/kg/day) for another 3 weeks.
|
| Growth protocol |
6 weeks old double floxed homozygous AMPK alpha 1 and alpha2 mice were fed on high-fat diet (60 % caloris) for 4 weeks. After the viral injection, mice were treated with metformin (50 mg/kg/day) for another three weeks before the termination of experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified by using Trizol, followed by Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled with the Affymetrix GeneChip WT PLUS Reagent Kit using the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
17hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Description |
Floxed a1/2-5
|
| Data processing |
Affymetrix CEL files were extracted with the Partek GS 6.6 platform and their data RMA and quantile-normalized to create log2 transcript signal values, which were used in subsequent ANOVA analysis. Transcript annotation was subsequently updated.
|
| |
|
| Submission date |
May 09, 2018 |
| Last update date |
Nov 13, 2019 |
| Contact name |
Ling He |
| Organization name |
The Johns Hopkins University School of Medicine
|
| Department |
Department of Pediatrics-Division of Endocrinology
|
| Street address |
600 North Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE114234 |
Importance of AMPK in metformin suppression of liver glucose production |
|
