|
| Status |
Public on Oct 01, 2019 |
| Title |
keratinocytes_si-Ctrl_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
keratinocytes transfected with si-control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin
cell type: Human primary epidermal keratinocytes (HEKa)
transfected with: 50 nM si-Control for 24hrs
|
| Treatment protocol |
The third passage keratinocytes at 60-70% confluence were transfected with 50 nM si-hsa_circ_0084443 or si Control (Eurofins Genomics) using Lipofectamine 2000 (Invitrogen).
|
| Growth protocol |
Human epidermal keratinocytes (HEKa) were cultured in EpiLife medium (Gibco™) supplemented with Human Keratinocyte Growth Supplement (Cascade Biologics) and 1% penicillin streptomycin (Gibco™) at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
24 hours after transfection, the total RNA was extracted with Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s (Affymetrix) instructions.
|
| |
|
| Hybridization protocol |
The samples were hybridized to a Clariom S Human Array Plate according to standard Affymetrix GeneTitan protocol.
|
| Scan protocol |
The array plate was scanned using the Affymetrix GeneTitan Scanner.
|
| Description |
MT1_si_ctrl_50nM-2_(Clariom_S_Human).CEL
|
| Data processing |
Generated CEL files were analyzed using Transcriptome Analysis Console (TAC) v. 4.0.1 and the RMA method
|
| |
|
| Submission date |
May 09, 2018 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
|
| Street address |
CMM L8:02
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE114236 |
A global transcriptome analysis of human epidermal keratinocytes upon knockdown of hsa_circ_0084443 |
|
