|
| Status |
Public on Apr 15, 2009 |
| Title |
Macrophages_MNVinfected_12h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
RAWcells, MNV-1 infected, 12hours
|
| Organism |
Mus musculus |
| Characteristics |
MNV-1, mouse norovirus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNeasy kit as described in the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
As described in Affymetrix's Eukaryotic Sample and Array Processing manual
|
| |
|
| Hybridization protocol |
As described in Affymetrix's Eukaryotic Sample and Array Processing manual
|
| Scan protocol |
As described in Affymetrix's Eukaryotic Sample and Array Processing manual
|
| Description |
The macrophage cell line RAW264.7 was infected with mouse norovirus MNV-1. The cells were harvested 12 hours post infection, and the expression profile of infected cells was compared to the expression profile of mock-infected cells.
|
| Data processing |
Data were analysed using GeneSpring GX 7.3.1 (Agilent, Santa Clara, CA). Signal values from the probe sets were calculated using micro array suite 5 (MAS5). Each chip was independently normalized to the median expression level of all genes on the chip. Each gene was then normalized to the median expression levels of that gene. Each of the 45,101 genes was filtered by the call of “present or marginal” Affymetrix flag in 3 of the sample replicates. The remaining genes were analyzed by 1-way ANOVA to identify those with statistically significant differences between samples infected with MNV-1 (3 replicates) and uninfected samples (3 replicates). Variances were calculated using the parametric test with p-value cutoff 0.05.
|
| |
|
| Submission date |
Aug 20, 2008 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Karin Bok |
| E-mail(s) |
bokk@niaid.nih.gov
|
| Phone |
301-594-1633
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
LID
|
| Street address |
50 South drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12518 |
Differential expression profile between MNV-1 infected and mock-infected RAW 264.7 cells. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
