|
| Status |
Public on May 16, 2018 |
| Title |
KRAS IKKa low cell, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
lung adenocarcinoma-associated macrophages from C57BL/6 mouse intratracheally injected with KRAS IKKa low cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6/J
genotype/variation: wild type
tissue: lung
treatment: Kras IKKa
|
| Treatment protocol |
The mice were intratracheally injected with Kras_CL or Kras IKKa low cells. At 1.5 months post injection, the mice were euthanized and lung tissues were collected for macrophage isolation.
|
| Growth protocol |
All mice used in this study were cared for in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC) of the National Institute of Health (NIH).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150ng total RNA (IVT Express 15402 or IVT Plus 18737)
|
| |
|
| Hybridization protocol |
15 ug of biotinylated cRNA were fragmented and hybridized for 16 hr at 45C on mouse Genome Array mo430 2.0.
|
| Scan protocol |
GeneChips were washed and stained in the Affymetrix Fluidics Station 450, were scanned using the Affymetrix Scanner 7G.
|
| Data processing |
The data were analyzed using Affymetrix Expression Console and normalized to 150.
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
Feb 01, 2019 |
| Contact name |
NA-YOUNG SONG |
| E-mail(s) |
na-young.song@nih.gov
|
| Organization name |
NCI
|
| Street address |
1050 Boyles Street
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE114501 |
Gene expression from mouse lung adenocarcinoma-associated macrophages |
|
