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Sample GSM3143174 Query DataSets for GSM3143174
Status Public on May 11, 2021
Title Fah-/- tumor rep1
Sample type SRA
 
Source name liver
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Fah-/-
age: 12 mohths
Treatment protocol Liver tissues were collected at defined points
Growth protocol WT liver(WT1-WT3) and Alb-Cre:Hdac3−/− liver (0d(Ha-Hc), 7d(Hd-Hg), 21d(Hh-Hk), 50d(Hl-Hn)) receiving successive PH(sPH), Alb-Cre:Hdac3−/− tumor tissues(HCC1-HCC3) and adjacent non-tumor tissues(N1-N3), spontaneous liver cancers of Alb-Cre:Hdac3-/- mice (HCC1-HCC3) and liver tumor of recipient Fah-/- mice (HCCa-HCCc)
Extracted molecule total RNA
Extraction protocol Livers were collected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model BGISEQ-500
 
Data processing The filtered data was compared with reference sequence by software HISAT and Bowtie2.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample.
 
Submission date May 15, 2018
Last update date May 11, 2021
Contact name ji hong jie
E-mail(s) 1403786850@qq.com
Organization name West China Hospital
Street address 37 Guoxue Road, Chengdu, China
City chengdou
ZIP/Postal code 610041
Country China
 
Platform ID GPL23479
Series (1)
GSE114503 Next Generation Sequencing Facilitates Quantitative Analysis of three groups from mouse liver Transcriptomes
Relations
BioSample SAMN09213266
SRA SRX4088692

Supplementary file Size Download File type/resource
GSM3143174_HCCa.xls.gz 235.3 Kb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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