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Sample GSM3149902 Query DataSets for GSM3149902
Status Public on Oct 26, 2018
Title stim_7h
Sample type RNA
 
Source name MNT-1 melanoma line
Organism Homo sapiens
Characteristics time: 7h
Treatment protocol MNT-1 melnaoma cells were serum depleted for 48 hours perior to treatment with 20uM Forskolin for 30 minutes, followed by replacment of medium with serum depleted medium. RNA was extracted at the indicated time points following stimulaiton.
Growth protocol MNT-1 melanoma cells were grown in 10% serum DMEM.
Extracted molecule total RNA
Extraction protocol RNA was isolated from MNT-1 cells using Trizol (Qiagen), according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with Clarioum S Human Arrays (Affymetrix) and scanned at the HUJI Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RNA expression value derived from Affymetrix Launcher software
Data processing Raw data were processed in R for background correction and RMA normalization. Normalized expression data were log-transformed.
 
Submission date May 22, 2018
Last update date Oct 26, 2018
Contact name Carmit Levy
E-mail(s) carmitlevy@post.tau.ac.il
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platform ID GPL23159
Series (1)
GSE114764 UV-protection timer controls a trade-off between skin protection systems

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
TC0100006437.hg.1 5.059631879
TC0100006476.hg.1 6.098025018
TC0100006479.hg.1 6.942404874
TC0100006480.hg.1 5.678315851
TC0100006483.hg.1 5.234541277
TC0100006486.hg.1 5.516239557
TC0100006490.hg.1 5.082337281
TC0100006492.hg.1 6.000952143
TC0100006494.hg.1 7.060999817
TC0100006497.hg.1 4.557569022
TC0100006499.hg.1 6.414737198
TC0100006501.hg.1 6.399492252
TC0100006502.hg.1 5.094465934
TC0100006514.hg.1 4.975570358
TC0100006516.hg.1 6.017316331
TC0100006517.hg.1 4.560187263
TC0100006524.hg.1 5.685113727
TC0100006540.hg.1 5.693504525
TC0100006548.hg.1 4.556913323
TC0100006550.hg.1 6.397577051

Total number of rows: 19577

Table truncated, full table size 571 Kbytes.




Supplementary file Size Download File type/resource
GSM3149902_03-24-6-7h_Clariom_S_Human_.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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