|
| Status |
Public on Oct 26, 2018 |
| Title |
stim_27h |
| Sample type |
RNA |
| |
|
| Source name |
MNT-1 melanoma line
|
| Organism |
Homo sapiens |
| Characteristics |
time: 27h
|
| Treatment protocol |
MNT-1 melnaoma cells were serum depleted for 48 hours perior to treatment with 20uM Forskolin for 30 minutes, followed by replacment of medium with serum depleted medium. RNA was extracted at the indicated time points following stimulaiton.
|
| Growth protocol |
MNT-1 melanoma cells were grown in 10% serum DMEM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from MNT-1 cells using Trizol (Qiagen), according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with Clarioum S Human Arrays (Affymetrix) and scanned at the HUJI Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RNA expression value derived from Affymetrix Launcher software
|
| Data processing |
Raw data were processed in R for background correction and RMA normalization. Normalized expression data were log-transformed.
|
| |
|
| Submission date |
May 22, 2018 |
| Last update date |
Oct 26, 2018 |
| Contact name |
Carmit Levy |
| E-mail(s) |
carmitlevy@post.tau.ac.il
|
| Organization name |
Tel Aviv University
|
| Department |
Human Genetics and Biochemistry
|
| Lab |
Sackler School of Medicine
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE114764 |
UV-protection timer controls a trade-off between skin protection systems |
|
