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Sample GSM315342 Query DataSets for GSM315342
Status Public on Jan 01, 2009
Title HG_adult_male_rep6_Cy3
Sample type RNA
 
Source name Harderian gland, adult male, Aoh2(-/-)
Organism Mus musculus
Characteristics Strain: C57BL/6NCrl
Gender: male
Age: 9 weeks
Tissue: Harderian gland
Eartag: 157
Aoh2 (-/-)
Treatment protocol Not applicable.
Growth protocol Not applicable.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNeasy kit, following manufacturer's instructions.
Label Cy3
Label protocol Amino Allyl Labeling Kit (cat 1705, Ambion), following manufacturer's instructions. Samples were then diluted 1:10 and 10 picomoles of each dye was used for hybridization.
 
Hybridization protocol Hybridization and washing were done according to Agilent protocol (SSPE).
Scan protocol Agilent Technologies Scanner G2505B US45102933, 10 micron resolution using the Agilent scan control software.
Description Gene expression of KO mouse.
Data processing Image data analysis was performed using the Feature extraction software (v9.1). Detailed description of the protocols used is reported in each raw data file. Vsn normalization (glog - please note: ln) was performed using the vsn package in Bioconductor.
 
Submission date Aug 25, 2008
Last update date Jan 04, 2012
Contact name Maddalena Fratelli
E-mail(s) maddalena@marionegri.it
Phone +390239014217
Organization name "Mario Negri" Institute
Lab Molecular Biology
Street address Via La Masa, 19
City Milano
ZIP/Postal code I20156
Country Italy
 
Platform ID GPL2872
Series (1)
GSE12541 Aldehyde oxidase homologue 2 (Aoh2) knockout mouse and retinoic acid synthesis

Data table header descriptions
ID_REF
VALUE vsn-normalized values

Data table
ID_REF VALUE
1 6.615653463
2 2.218162695
3 4.837559786
4 4.359608993
5 2.627653731
6 2.447790793
7 6.782595546
8 2.2765361
9 2.959206137
10 5.296661638
11 2.793610661
12 2.447790793
13 2.410375063
14 6.951859938
15 2.179044013
16 2.372556444
17 5.312836759
18 2.237668257
19 2.503077012
20 6.780745833

Total number of rows: 43790

Table truncated, full table size 753 Kbytes.




Supplementary file Size Download File type/resource
GSM315342_US45102933_251269418345_S01_GE2-SSPE_91_2-local_noSI.txt 33.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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