|
| Status |
Public on Jan 01, 2009 |
| Title |
Skin_adult_female_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Skin, adult female, wild type
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6NCrl
Gender: female
Age: 8 weeks
Tissue: Skin
Eartag: 705
wild type
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy kit, following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Amino Allyl Labeling Kit (cat 1705, Ambion), following manufacturer's instructions. Samples were then diluted 1:10 and 10 picomoles of each dye was used for hybridization.
|
| |
|
| Channel 2 |
| Source name |
Skin, adult female, Aoh2 (-/-)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6NCrl
Gender: female
Age: 8 weeks
Tissue: Skin
Eartag: 708
Aoh2 (-/-)
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy kit, following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl Labeling Kit (cat 1705, Ambion), following manufacturer's instructions. Samples were then diluted 1:10 and 10 picomoles of each dye was used for hybridization.
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing were done according to Agilent protocol (GE version 5.5).
|
| Scan protocol |
Agilent Technologies Scanner G2505B US45102933, 5 micron resolution and XDR protocol using the Agilent scan control software.
|
| Description |
Gene expression of WT vs. KO mouse.
|
| Data processing |
Image data analysis was performed using the Feature extraction software (v9.1). Detailed description of the protocols used is reported in each raw data file. Log ratios are derived by the 'Per Spot and Per Chip: Intensity dependent (Lowess) normalization' of the Genespring GX 7.3 software (Agilent Technologies).
|
| |
|
| Submission date |
Aug 25, 2008 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Maddalena Fratelli |
| E-mail(s) |
maddalena@marionegri.it
|
| Phone |
+390239014217
|
| Organization name |
"Mario Negri" Institute
|
| Lab |
Molecular Biology
|
| Street address |
Via La Masa, 19
|
| City |
Milano |
| ZIP/Postal code |
I20156 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE12541 |
Aldehyde oxidase homologue 2 (Aoh2) knockout mouse and retinoic acid synthesis |
|
