|
Status |
Public on May 30, 2018 |
Title |
Primary tumor tissue of patient 3 PrE-3 |
Sample type |
RNA |
|
|
Source name |
Macrodissected primary prostate tumor
|
Organism |
Homo sapiens |
Characteristics |
cell type: Tissue
|
Treatment protocol |
PrE dervied organoids were cultured with or without PrS cells
|
Growth protocol |
Primary prostate epithelial (PrE) and stromal (PrS) cells were grown independently in 2D, mixed, and seeded into 3D matrigel and cultured for 7 to 14 days prior to harvest
|
Extracted molecule |
total RNA |
Extraction protocol |
Matrigel from 3D cultures was dissociated, cells were trypsinized to form single cell suspension and sorted by fluorescent associated cell sorting according to EpCAM. RNA extracted using the TRIzol method.
|
Label |
Biotin
|
Label protocol |
Labeling followed the GeneChip Pico Reagent Kit (Affymetrix) protocol
|
|
|
Hybridization protocol |
Hybridization followed the GeneChip Pico Reagent Kit (Affymetrix) protocol
|
Scan protocol |
standard Affymetrix protocol
|
Description |
Gene expression data from FACS sorted co-culture Primary tumor tissue of PrE-3
|
Data processing |
Data were processed using the Transcriptome Analysis Console (TAC) Software (Thermo Fisher Scientific)
|
|
|
Submission date |
May 29, 2018 |
Last update date |
May 30, 2018 |
Contact name |
Larisa Nonn |
E-mail(s) |
lnonn@uic.edu
|
Phone |
3129960194
|
Organization name |
University of Illinois at Chicago
|
Department |
Pathology
|
Street address |
840 S. Wood St, MC847
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60612 |
Country |
USA |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE115052 |
Primary prostate organoid transcriptomic profile in co-culture with primary prostate stroma |
|