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Sample GSM3164181 Query DataSets for GSM3164181
Status Public on May 30, 2018
Title Primary tumor tissue of patient 3 PrE-3
Sample type RNA
 
Source name Macrodissected primary prostate tumor
Organism Homo sapiens
Characteristics cell type: Tissue
Treatment protocol PrE dervied organoids were cultured with or without PrS cells
Growth protocol Primary prostate epithelial (PrE) and stromal (PrS) cells were grown independently in 2D, mixed, and seeded into 3D matrigel and cultured for 7 to 14 days prior to harvest
Extracted molecule total RNA
Extraction protocol Matrigel from 3D cultures was dissociated, cells were trypsinized to form single cell suspension and sorted by fluorescent associated cell sorting according to EpCAM. RNA extracted using the TRIzol method.
Label Biotin
Label protocol Labeling followed the GeneChip Pico Reagent Kit (Affymetrix) protocol
 
Hybridization protocol Hybridization followed the GeneChip Pico Reagent Kit (Affymetrix) protocol
Scan protocol standard Affymetrix protocol
Description Gene expression data from FACS sorted co-culture
Primary tumor tissue of PrE-3
Data processing Data were processed using the Transcriptome Analysis Console (TAC) Software (Thermo Fisher Scientific)
 
Submission date May 29, 2018
Last update date May 30, 2018
Contact name Larisa Nonn
E-mail(s) lnonn@uic.edu
Phone 3129960194
Organization name University of Illinois at Chicago
Department Pathology
Street address 840 S. Wood St, MC847
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
 
Platform ID GPL23159
Series (1)
GSE115052 Primary prostate organoid transcriptomic profile in co-culture with primary prostate stroma

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
TC0100006437.hg.1 4.54
TC0100006476.hg.1 4.62
TC0100006479.hg.1 6.28
TC0100006480.hg.1 7.07
TC0100006483.hg.1 9.14
TC0100006486.hg.1 9.96
TC0100006490.hg.1 4.12
TC0100006492.hg.1 5.64
TC0100006494.hg.1 5.26
TC0100006497.hg.1 4.36
TC0100006499.hg.1 6.65
TC0100006501.hg.1 4.44
TC0100006502.hg.1 4.56
TC0100006514.hg.1 4.13
TC0100006516.hg.1 5.04
TC0100006517.hg.1 3.06
TC0100006524.hg.1 7.42
TC0100006540.hg.1 5.46
TC0100006548.hg.1 3.54
TC0100006550.hg.1 7.65

Total number of rows: 21448

Table truncated, full table size 485 Kbytes.




Supplementary file Size Download File type/resource
GSM3164181_Primary_tumor_tissue_of_PrE-3.CEL.gz 957.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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