|
| Status |
Public on May 31, 2018 |
| Title |
Harumi_45_DAF |
| Sample type |
RNA |
| |
|
| Source name |
Harumi, 45 day after flowering, polyembryonic
|
| Organism |
(Citrus unshiu x Citrus sinensis) x Citrus reticulata |
| Characteristics |
cultivar: Harumi
tissue: whole young fruit
embryonic: polyembryonic
|
| Treatment protocol |
Whole young fruits at 15, 30, 45 and 60 DAF were collected for microarray analysis, immediately frozen in liquid nitrogen, and stored at −80°C for RNA isolation.
|
| Growth protocol |
‘Kiyomi’ and ‘Harumi’, cultivated at NARO Institute of Fruit and Tea Tree Science (NIFTS), Department of Citrus Research (Shizuoka, Japan), were used as materials for monoembryonic and polyembryonic varieties, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted following Ikoma et al. (1996) J. Jpn. Soc. Hort. Sci. 64: 809-814.
|
| Label |
Cy3
|
| Label protocol |
RNA samples were labelled with cyanine-3-labelled cytosine triphosphate using a Low Input Quick-Amp Labelling Kit (Agilent Technologies) according to the manufacturer’s directions.
|
| |
|
| Hybridization protocol |
The labelled cRNAs were subsequently hybridized on the custom oligo-DNA microarray using a Gene Expression Hybridization kit (Agilent Technologies) according to the manufacturer’s directions.
|
| Scan protocol |
After washing in GE washing buffer (Agilent Technologies), the glass slides were scanned with an Agilent Microarray Scanner (G2505C, Agilent Technologies).
|
| Description |
Gene expression 45 day after flowering in whole young fruit of polyembryonic citrus cultivar 'Harumi'
|
| Data processing |
The Feature Extraction software (version 10.5.1.1), employing defaults for all parameters, was used to convert the images into gene expression data.
|
| |
|
| Submission date |
May 30, 2018 |
| Last update date |
May 31, 2018 |
| Contact name |
Hiroshi Fujii |
| E-mail(s) |
hfujii@affrc.go.jp
|
| Organization name |
Institute of Fruit Tree Science, NARO
|
| Department |
Division of Citrus Research
|
| Street address |
485-6 Okitsunakacho, Shimizu
|
| City |
Shizuoka |
| State/province |
Shizuoka |
| ZIP/Postal code |
424-0292 |
| Country |
Japan |
| |
|
| Platform ID |
GPL25057 |
| Series (1) |
| GSE115082 |
MITE insertion-dependent expression of CitRKD1 with a RWP-RK domain regulates somatic embryogenesis in citrus nucellar tissues |
|
