NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM317232 Query DataSets for GSM317232
Status Public on Sep 01, 2009
Title 0320-a20
Sample type RNA
 
Source name myocardium, the remote zone tissues of left ventricular free wall, STZ
Organism Rattus norvegicus
Characteristics Strain: Sprague-Dawley rats
Gender: male
Age: 20 weeks
Tissue: myocardium, the remote zone tissues of left ventricular free wall post AMI
Biomaterial provider obtained from laboratorial animal center of Chinese University of Agriculture
Treatment protocol When the rats is 8 weeks old, STZ 65mg/kg, single intraperitoneal injection, then they were raised without blood sugar control for 10 weeks before the ligation of left anterior descending coronary artery.
Growth protocol When the rats is 8 weeks old, STZ 65mg/kg, single intraperitoneal injection, then they were raised without blood sugar control for 10 weeks before the ligation of left anterior descending coronary artery, and then they were again raised without blood sugar control for 2 weeks after the ligation before sacrifice.
Extracted molecule total RNA
Extraction protocol available at manufacturer's web site
www.affymetrix.com
Label biotin
Label protocol available at manufacturer's web site
www.affymetrix.com
 
Hybridization protocol available at manufacturer's web site
www.affymetrix.com
Scan protocol available at manufacturer's web site
www.affymetrix.com
Description According to previous studies, we used the sample pooling strategies for microarray analysis in order to reduce the whole cost of the study. 3 samples were randomly chosen from each time point, and then total RNA was isolated from the frozen tissue fragments using TRIzol (Invitrogen). After the ethanol precipitation step in the TRIzol extraction procedure, we performed a cleanup using the QIAGEN RNeasy Mini Kit. The RNA fractions from the three rats in each group at the time point were balanced pooled for GeneChip analysis.
Data processing Algorithm: Expression Console PLIER, available at manufacturer's web site
www.affymetrix.com
 
Submission date Sep 01, 2008
Last update date Oct 16, 2008
Contact name guangyuan song
E-mail(s) canghailinghu@yahoo.com.cn
Organization name Cardiovascular Institute & Fu-Wai Hospital
Department Center of Coronary Heart Disease
Street address 167 BeiLiShi Rd, Beijing
City beijing
ZIP/Postal code 100037
Country China
 
Platform ID GPL1355
Series (1)
GSE12639 diabetes mellitus and the progression of post-infarction genetic regulatory expression

Data table header descriptions
ID_REF
VALUE signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 100.003
AFFX-BioB-M_at 142.586
AFFX-BioB-3_at 117.871
AFFX-BioC-5_at 293.849
AFFX-BioC-3_at 347.092
AFFX-BioDn-5_at 760.656
AFFX-BioDn-3_at 1382.9
AFFX-CreX-5_at 3685.76
AFFX-CreX-3_at 4046.83
AFFX-DapX-5_at 392.196
AFFX-DapX-M_at 895.24
AFFX-DapX-3_at 1214.26
AFFX-LysX-5_at 61.5863
AFFX-LysX-M_at 94.2055
AFFX-LysX-3_at 232.054
AFFX-PheX-5_at 92.8912
AFFX-PheX-M_at 152.977
AFFX-PheX-3_at 157.524
AFFX-ThrX-5_at 122.653
AFFX-ThrX-M_at 193.727

Total number of rows: 31099

Table truncated, full table size 578 Kbytes.




Supplementary file Size Download File type/resource
GSM317232.CEL.gz 2.3 Mb (ftp)(http) CEL
GSM317232.chp.gz 209.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap