|
| Status |
Public on Sep 01, 2009 |
| Title |
0320-CA |
| Sample type |
RNA |
| |
|
| Source name |
myocardium, the remote zone tissues of left ventricular free wall, sodium citrate buffer
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Sprague-Dawley rats
Gender: male
Age: 18 weeks
Tissue: myocardium, the remote zone tissues of left ventricular free wall post AMI
|
| Biomaterial provider |
obtained from laboratorial animal center of Chinese University of Agriculture
|
| Treatment protocol |
When the rats is 8 weeks old, the same dose of sodium citrate buffer, single intraperitoneal injection, then they were raised without blood sugar control for 10 weeks before the sham operation.
|
| Growth protocol |
When the rats is 8 weeks old, the same dose of sodium citrate buffer, single intraperitoneal injection, then they were raised without blood sugar control for 10 weeks before the sham operation, and then they were sacrificed for microarray analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
available at manufacturers's web site
www.affymetrix.com
|
| Label |
biotin
|
| Label protocol |
available at manufacturers's web site
www.affymetrix.com
|
| |
|
| Hybridization protocol |
available at manufacturers's web site
www.affymetrix.com
|
| Scan protocol |
available at manufacturers's web site
www.affymetrix.com
|
| Description |
According to previous studies, we used the sample pooling strategies for microarray analysis in order to reduce the whole cost of the study. 3 samples were randomly chosen from each time point, and then total RNA was isolated from the frozen tissue fragments using TRIzol (Invitrogen). After the ethanol precipitation step in the TRIzol extraction procedure, we performed a cleanup using the QIAGEN RNeasy Mini Kit. The RNA fractions from the three rats in each group at the time point were balanced pooled for GeneChip analysis.
|
| Data processing |
Algorithm: Expression Console PLIER available at manufacturer's web site
www.affymetrix.com
|
| |
|
| Submission date |
Sep 01, 2008 |
| Last update date |
Oct 16, 2008 |
| Contact name |
guangyuan song |
| E-mail(s) |
canghailinghu@yahoo.com.cn
|
| Organization name |
Cardiovascular Institute & Fu-Wai Hospital
|
| Department |
Center of Coronary Heart Disease
|
| Street address |
167 BeiLiShi Rd, Beijing
|
| City |
beijing |
| ZIP/Postal code |
100037 |
| Country |
China |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE12639 |
diabetes mellitus and the progression of post-infarction genetic regulatory expression |
|
