|
| Status |
Public on May 29, 2019 |
| Title |
T24_siG9a_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
T24_siG9a_48 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T24
cell line: bladder cancer cells
treatment: G9a siRNA
|
| Treatment protocol |
T24 cells were seeded in 6-well plates at 30% conference. After 24 h, cells were transfected with negative control siRNA or G9a siRNA using Lipofectamine RNAiMAX. Then, the cells were cultured for another 48 h and then prepared using Trizol.
|
| Growth protocol |
T24 cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (GeneChipTM WT PLUS Reagent Kit, 2017, Affymetrix)
|
| |
|
| Hybridization protocol |
Following Fragment and Label Single-Stranded cDNA, 27ul of biotin-labeled ss-cDNA sample were hybridized for 16 hr at 45℃ on GeneChip Clariom S Human Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G.
|
| Description |
T24-siG9a-3
|
| Data processing |
The data were analyzed with robust multi-array average (RMA) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 05, 2018 |
| Last update date |
May 29, 2019 |
| Contact name |
Jingya Sun |
| E-mail(s) |
sunjingya123@126.com
|
| Organization name |
Shanghai Institute of Materia Medica, Chinese Academy of Sciences
|
| Street address |
555 Zuchongzhi Road
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE115341 |
Expression data from bladder cancer cells T24 - treated with G9a siRNA or negative control siRNA |
|
