|
Status |
Public on Mar 24, 2021 |
Title |
Ctrl2 |
Sample type |
SRA |
|
|
Source name |
CD8+ T cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: LN genotype: OT-I treatment: control
|
Treatment protocol |
OT-I CD8+ T cells were then treated with control or 1 mM NaBu for 12 hr.
|
Growth protocol |
OT-I CD8+ T cells were purified by MACS negative selection to obtain purities >95% and cultured in complete RPMI-1640 culture medium with 10 ng/ml recombinant mouse IL-2 in plate pre-coated with 1 μg/ml anti-CD3 and 2 μg/ml anti-CD28 for 2 d. Cells were then washed once and cultured in a new plate with 10 ng/ml recombinant mouse IL-2 for another 2 d.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with the RNeasy Micro Kit (QIAGEN) RNA libraries were prepared for sequencing using standard protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
BGISEQ-500 |
|
|
Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie2 FPKM were calculated using RSEM tool Genome_build: mm10 Supplementary_files_format_and_content: xls files include FPKM values for each Sample
|
|
|
Submission date |
Jun 06, 2018 |
Last update date |
Mar 24, 2021 |
Contact name |
Liuhui Fu |
E-mail(s) |
fuliuhui27@163.com
|
Phone |
15056022224
|
Organization name |
Tsinghua university
|
Department |
Institute for Immunology
|
Lab |
Guo Lab
|
Street address |
Zhongguancun North Street
|
City |
Beijing |
State/province |
- |
ZIP/Postal code |
100084 |
Country |
China |
|
|
Platform ID |
GPL23479 |
Series (1) |
GSE115426 |
RNA-seq of Ctrl and NaBu-treated CD8+ T cells in vitro |
|
Relations |
BioSample |
SAMN09375635 |
SRA |
SRX4178354 |