|
| Status |
Public on Mar 24, 2021 |
| Title |
Ctrl2 |
| Sample type |
SRA |
| |
|
| Source name |
CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: LN
genotype: OT-I
treatment: control
|
| Treatment protocol |
OT-I CD8+ T cells were then treated with control or 1 mM NaBu for 12 hr.
|
| Growth protocol |
OT-I CD8+ T cells were purified by MACS negative selection to obtain purities >95% and cultured in complete RPMI-1640 culture medium with 10 ng/ml recombinant mouse IL-2 in plate pre-coated with 1 μg/ml anti-CD3 and 2 μg/ml anti-CD28 for 2 d. Cells were then washed once and cultured in a new plate with 10 ng/ml recombinant mouse IL-2 for another 2 d.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the RNeasy Micro Kit (QIAGEN)
RNA libraries were prepared for sequencing using standard protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie2
FPKM were calculated using RSEM tool
Genome_build: mm10
Supplementary_files_format_and_content: xls files include FPKM values for each Sample
|
| |
|
| Submission date |
Jun 06, 2018 |
| Last update date |
Mar 24, 2021 |
| Contact name |
Liuhui Fu |
| E-mail(s) |
fuliuhui27@163.com
|
| Phone |
15056022224
|
| Organization name |
Tsinghua university
|
| Department |
Institute for Immunology
|
| Lab |
Guo Lab
|
| Street address |
Zhongguancun North Street
|
| City |
Beijing |
| State/province |
- |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE115426 |
RNA-seq of Ctrl and NaBu-treated CD8+ T cells in vitro |
|
| Relations |
| BioSample |
SAMN09375635 |
| SRA |
SRX4178354 |
