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Sample GSM318173 Query DataSets for GSM318173
Status Public on Nov 25, 2008
Title Patient6_339_GH_Day1_GH0_8PM
Sample type RNA
 
Source name Day1_GH0_8PM
Organism Homo sapiens
Characteristics Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 g/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description 339_GH_004
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Sep 04, 2008
Last update date Nov 25, 2008
Contact name Patricia Maningat
E-mail(s) mmaningat@mail.rockefeller.edu
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE12669 Gene expression in the human mammary epithelium during lactation: the milk fat globule transcriptome

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 127.2101 42 5.783703 0.004237288
ILMN_1660305 271.8036 39 8.538553 0
ILMN_1792173 198.7935 33 7.005133 0.001412429
ILMN_1762337 68.26745 47 2.682129 0.2485876
ILMN_1736007 66.68565 56 2.078698 0.319209
ILMN_1787689 66.96917 54 2.243516 0.309322
ILMN_1731507 54.12002 30 2.418023 0.9293785
ILMN_1814316 67.62244 36 2.730478 0.279661
ILMN_1745607 59.82008 45 2.925418 0.6694915
ILMN_1757454 64.41901 53 2.303396 0.4322034
ILMN_1735045 68.76995 40 2.849471 0.2231638
ILMN_1680754 70.90565 40 2.968571 0.1511299
ILMN_1659452 62.06906 34 2.121059 0.5480226
ILMN_1767388 103.6274 45 3.353742 0.01129944
ILMN_1673870 62.35218 33 2.553711 0.539548
ILMN_1675204 65.72167 46 2.486351 0.358757
ILMN_1755321 137.2515 51 4.072211 0.001412429
ILMN_1698554 1241.896 45 29.00027 0
ILMN_1805938 61.60796 34 2.771255 0.5677966
ILMN_1676336 70.48264 52 2.081141 0.1624294

Total number of rows: 22184

Table truncated, full table size 909 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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