NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM318188 Query DataSets for GSM318188
Status Public on Nov 25, 2008
Title Patient5_339_GH_Day1_GH0_5PM
Sample type RNA
 
Source name Day1_GH0_5PM
Organism Homo sapiens
Characteristics Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 g/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description 339_GH_059
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Sep 04, 2008
Last update date Nov 25, 2008
Contact name Patricia Maningat
E-mail(s) mmaningat@mail.rockefeller.edu
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE12669 Gene expression in the human mammary epithelium during lactation: the milk fat globule transcriptome

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 123.1078 46 4.674443 0.001412429
ILMN_1660305 252.3946 27 11.06551 0
ILMN_1792173 189.2043 32 6.928622 0
ILMN_1762337 76.39279 47 2.852259 0.0579096
ILMN_1736007 59.44856 36 2.439392 0.7146893
ILMN_1787689 75.33862 44 3.579864 0.06355932
ILMN_1731507 53.90356 32 2.270499 0.9449152
ILMN_1814316 67.00161 35 3.462957 0.2966102
ILMN_1745607 65.02203 43 2.987634 0.3827684
ILMN_1757454 67.55923 39 3.38471 0.2782486
ILMN_1735045 77.32291 43 3.385764 0.04661017
ILMN_1680754 73.24107 41 3.43117 0.1016949
ILMN_1659452 59.59426 32 2.690765 0.7048023
ILMN_1767388 103.1498 39 4.745258 0.008474576
ILMN_1673870 61.8247 31 3.701628 0.5635593
ILMN_1675204 60.89103 39 3.026596 0.6214689
ILMN_1755321 158.4777 42 5.37923 0.001412429
ILMN_1698554 937.4144 52 28.24296 0
ILMN_1805938 60.63145 41 2.717862 0.6313559
ILMN_1676336 95.11588 49 3.312129 0.01129944

Total number of rows: 22184

Table truncated, full table size 904 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap