NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3188481 Query DataSets for GSM3188481
Status Public on Jun 21, 2019
Title Hipp IR 3
Sample type RNA
 
Source name Hipp from mouse 3 receiving cranial radiation
Organism Mus musculus
Characteristics tissue: hippocampus
treatment: radiation
Treatment protocol Frozen tissues were used for RNA isolation
Growth protocol Hipp and PLv from mouse brains were isolated and immediately frozen in liquid nitrogen and stored at -80 degrees C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNAeasy Mini kit (Qiagen) following the instructions of the manufacturer.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 2ng total RNA Labeling Protocol (Manual Target Preparation for GeneChip® Whole Transcript (WT) Expression ArraysWT Pico Reagent Kit, ©2015 Affymetrix Inc.)
 
Hybridization protocol Following fragmentation, 2.3 µg of single-stranded cDNA were hybridized for 16 hr at 45ºC on Clariom™ S Assay Mouse Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
Scan protocol Microarrays were scanned using GeneChip® Scanner 3000 7G
Description Gene expression data from the Hipp isolated from mice receiving radiation
Data processing The .CEL files were generated using Affymetrix AGCC software and further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis (ANOVA unpaired one-way) were performed using the TAC software (Transcriptome Analysis Console) developed by Affymetrix.
 
Submission date Jun 13, 2018
Last update date Jun 21, 2019
Contact name Vivian Capilla-Gonzalez
Organization name CABIMER
Street address Avda. Americo Vespucio 24
City SEVILLA
State/province Sevilla
ZIP/Postal code 41092
Country Spain
 
Platform ID GPL23038
Series (1)
GSE115735 Human mesenchymal stem cells prevent neurological complications of radiotherapy

Data table header descriptions
ID_REF
VALUE log2-rma
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
AFFX-BkGr-GC03_st 6.70661 0.794128
AFFX-BkGr-GC04_st 6.38149 0.96834
AFFX-BkGr-GC05_st 6.61009 0.988167
AFFX-BkGr-GC06_st 6.30067 0.993392
AFFX-BkGr-GC07_st 6.37885 0.99183
AFFX-BkGr-GC08_st 4.74759 0.983859
AFFX-BkGr-GC09_st 4.31021 0.972223
AFFX-BkGr-GC10_st 3.60589 0.954597
AFFX-BkGr-GC11_st 3.15038 0.929517
AFFX-BkGr-GC12_st 2.62281 0.867081
AFFX-BkGr-GC13_st 2.25888 0.915442
AFFX-BkGr-GC14_st 2.08586 0.909421
AFFX-BkGr-GC15_st 2.10372 0.906793
AFFX-BkGr-GC16_st 2.26974 0.868501
AFFX-BkGr-GC17_st 2.3419 0.834798
AFFX-BkGr-GC18_st 2.79626 0.751277
AFFX-BkGr-GC19_st 5.70277 0.607012
AFFX-BkGr-GC20_st 5.92539 0.603373
AFFX-BkGr-GC21_st 5.9864 0.596319
AFFX-BkGr-GC22_st 6.05774 0.591441

Total number of rows: 28846

Table truncated, full table size 1055 Kbytes.




Supplementary file Size Download File type/resource
GSM3188481_Vivian6_R83-HP_Clariom_S_Mouse_.CEL.gz 1.1 Mb (ftp)(http) CEL
GSM3188481_Vivian6_R83-HP_Clariom_S_Mouse_.sst-rma-gene-full.chp.gz 320.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap