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Sample GSM3191804 Query DataSets for GSM3191804
Status Public on Jun 01, 2020
Title Patien4_recFSH
Sample type RNA
 
Source name hGCs_recFSH_24h
Organism Homo sapiens
Characteristics tissue: Primary culture of human granulosa cells (hGCs)
Treatment protocol hGCs primary cultures were maintained in starvation buffer for 12 hours. hGCs were treated with bioequivalent doses of corifollitrophin alfa or rec FSH. hGCs were evaluated after 24 hours from the removal of the unbound gonadotrophins.
Growth protocol hGCs were recovered from preovulatory ovarian follicles after the oocytes retrieval from the medium aspirated. Retrieved hGCs were cultured for 6 days in McCoy 5A medium (Carlo Erba, Italy) supplemented with 5% foetal bovine serum (FBS) South America (EU Approved, Carlo Erba, Italy), 2mM L-Glutamine, 1% Penicillin/Streptomicin and 1% Amphotericin B (Sigma Aldrich, St. Louis, MO, USA) at 37 °C under a controlled atmosphere of 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer's protocol
Label biotin
Label protocol not provided
 
Hybridization protocol not provided
Scan protocol not provided
Data processing affymetrix-algorithm-name = sst-rma-gene-full
program-name = Expression Console
program-version = 1.4.1.46
 
Submission date Jun 15, 2018
Last update date Jun 01, 2020
Contact name Antonio La Marca
E-mail(s) antlamarca@libero.it
Phone +390594224671
Organization name University of Modena and Reggio
Department Mother-Infant Department, Institute of Obstetrics and Gynecology
Street address Via del pozzo, 71
City Modena
State/province Modena
ZIP/Postal code 41100
Country Italy
 
Platform ID GPL23126
Series (1)
GSE115846 Gene Expression Profiles of Human Granulosa Cells Treated With Bioequivalent Doses of Corifollitrophin Alfa or Recombinant Human Follicle-Stimulating Hormone

Data table header descriptions
ID_REF
VALUE log2 RMA

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 8.56928
AFFX-BkGr-GC04_st 7.1743
AFFX-BkGr-GC05_st 6.513
AFFX-BkGr-GC06_st 5.32355
AFFX-BkGr-GC07_st 4.633
AFFX-BkGr-GC08_st 4.0772
AFFX-BkGr-GC09_st 3.65064
AFFX-BkGr-GC10_st 3.38497
AFFX-BkGr-GC11_st 3.02772
AFFX-BkGr-GC12_st 2.72391
AFFX-BkGr-GC13_st 2.43283
AFFX-BkGr-GC14_st 2.27786
AFFX-BkGr-GC15_st 2.15264
AFFX-BkGr-GC16_st 2.2008
AFFX-BkGr-GC17_st 2.09959
AFFX-BkGr-GC18_st 2.23355
AFFX-BkGr-GC19_st 2.48807
AFFX-BkGr-GC20_st 2.52236
AFFX-BkGr-GC21_st 2.59851
AFFX-BkGr-GC22_st 2.9719

Total number of rows: 138745

Table truncated, full table size 3533 Kbytes.




Supplementary file Size Download File type/resource
GSM3191804_SAMPLE_12_WNDP_PUREGON_Clariom_D_Human.CEL.gz 24.6 Mb (ftp)(http) CEL
GSM3191804_SAMPLE_12_WNDP_PUREGON_Clariom_D_Human._SST_RMA.sst-rma-gene-full.chp.gz 886.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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