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Status |
Public on May 15, 2019 |
Title |
Vehicle_mouse_5 |
Sample type |
RNA |
|
|
Source name |
B16OVA tumors intratumorally injected with Vehicle
|
Organism |
Mus musculus |
Characteristics |
tissue: B16OVA tumor
|
Treatment protocol |
Intratumoral BO-112 or identical volumes of vehicle (5%glucose)
|
Growth protocol |
5x10^5 B16-OVA cells line were subcutaneously injected in B6 mice
|
Extracted molecule |
total RNA |
Extraction protocol |
Tumors were excissed, Total RNA was isolated in two steps using TRIzol (Life technologies) and Rneasy Mini-Kit (Quiagen) purification, following manufacturer`s RNA cleanup protocol.
|
Label |
Biotin
|
Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
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|
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Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix Mouse Clariom S array according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
|
Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
|
Description |
Gene expression data from whole subcutaneos B16OVA tumors
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Data processing |
Data was normalized using Bioconductor software and RMA algorithm.
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|
|
Submission date |
Jun 20, 2018 |
Last update date |
May 15, 2019 |
Contact name |
Maria Angela Aznar |
E-mail(s) |
maznargo@alumni.unav.es
|
Organization name |
Center for applied medical research (CIMA)
|
Street address |
Pio XII num 55
|
City |
Pamplona |
ZIP/Postal code |
E-31008 |
Country |
Spain |
|
|
Platform ID |
GPL23038 |
Series (2) |
GSE116074 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C (B16OVA tumors) |
GSE116078 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C |
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