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Sample GSM3214215 Query DataSets for GSM3214215
Status Public on Feb 20, 2019
Title TTFs-2_GHMT/EP4 antagonist
Sample type RNA
 
Source name GHMT transduced TTFs with EP4 antagonist
Organism Mus musculus
Characteristics cell type: postnatal tail-tip fibroblasts (TTFs)
treated with: EP4 antagonist
Growth protocol Fibroblasts were transduced with the retrovirus mixtures as indicated and DMSO, diclofenac, EP4 antagonist, PGE2, TGFb inhibitor or Wnt inhibitor was added simultaneously. The medium was replaced with FBS medium, which contained DMEM (high glucose) with L-glutamate and phenol red (Wako, Tokyo, Japan, 044-29765); Medium199 with Earle’s Salts, L-glutamate and 22 g/L sodium bicarbonate (Gibco, 11150-059); and 10% Hyclone Characterized FBS (Thermo Scientific, SV30014.03), after 24 h of infection and changed every 3 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted from GHMT-, GHMT/diclofenac-, GHMT/EP4 antagonist-, GHMT/diclofenac/PGE2-, GHMT/TGFb inhibitor- or GHMT/Wnt inhibitor-induced TTFs using the ReliaPrep RNA Cell Miniprep System (Promega, Z6012) quantified using a NanoDrop-2000c spectrophotometer. Quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label biotin
Label protocol Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip WT PLUS Reagent Kit User Manual, Affymetrix).Biotinylated ss-cDNA yield were checked with the NanoDrop ND-2000c Spectrophotometer.
 
Hybridization protocol Fragmented and labeled ss-cDNA were hybridized for 16 hr at 45°C on GeneChip Clariom S Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description SAMPLE 6
Data processing Raw data were processed with Affymetrix Expression Console software version 1.4 for normalization.
 
Submission date Jun 25, 2018
Last update date Feb 20, 2019
Contact name Naoto Muraoka
Organization name Keio University School of Medicine
Department Department of Cardiology
Street address 35 Shinanomachi, Shinjuku-ku
City Tokyo
ZIP/Postal code 1608582
Country Japan
 
Platform ID GPL23038
Series (1)
GSE116223 Enhanced cardiac reprogramming by Inhibition of cyclooxygenase-2/prostaglandin E2/prostaglandin E receptor 4 signaling

Data table header descriptions
ID_REF
VALUE log2 SST-RMA signal

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 6.59
AFFX-BkGr-GC04_st 5.75
AFFX-BkGr-GC05_st 5.83
AFFX-BkGr-GC06_st 5.65
AFFX-BkGr-GC07_st 4.83
AFFX-BkGr-GC08_st 3.96
AFFX-BkGr-GC09_st 3.48
AFFX-BkGr-GC10_st 3.29
AFFX-BkGr-GC11_st 3.01
AFFX-BkGr-GC12_st 2.79
AFFX-BkGr-GC13_st 2.59
AFFX-BkGr-GC14_st 2.44
AFFX-BkGr-GC15_st 2.43
AFFX-BkGr-GC16_st 2.53
AFFX-BkGr-GC17_st 2.6
AFFX-BkGr-GC18_st 2.99
AFFX-BkGr-GC19_st 4.98
AFFX-BkGr-GC20_st 5.15
AFFX-BkGr-GC21_st 5.09
AFFX-BkGr-GC22_st 5.24

Total number of rows: 28846

Table truncated, full table size 669 Kbytes.




Supplementary file Size Download File type/resource
GSM3214215_TTFs-2_GHMT_EP4_antagonist_Clariom_S_Mouse_.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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