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Sample GSM322065 Query DataSets for GSM322065
Status Public on Sep 18, 2008
Title Zebrafish_wt_24hpf, biological rep1
Sample type RNA
 
Source name Zebrafish embryo harvested at 24hpf
Organism Danio rerio
Characteristics Zebrafish wild-type whole-embryo RNA
Treatment protocol Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to manufacturer's (Affymetrix) recommendations
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description Gene expression data from wild-type embryos at 24 hours post fertilization
Data processing Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
 
Submission date Sep 17, 2008
Last update date Sep 17, 2008
Contact name Nathan Salomonis
E-mail(s) nathan.salomonis@cchmc.org
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1319
Series (1)
GSE12826 Zebrafish 24hpf mutant sk8 gene expression

Data table header descriptions
ID_REF
VALUE GC-RMA calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.657455654
AFFX-BioB-5_at 6.061548358
AFFX-BioB-M_at 6.713528905
AFFX-BioC-3_at 7.646404399
AFFX-BioC-5_at 7.628055787
AFFX-BioDn-3_at 9.913403571
AFFX-BioDn-5_at 9.252696
AFFX-CreX-3_at 12.96436297
AFFX-CreX-5_at 12.11838243
AFFX-DapX-3_at 7.667376427
AFFX-DapX-5_at 6.362178726
AFFX-DapX-M_at 6.998663567
AFFX-Dr-AB076373-1_at 1.974541149
AFFX-Dr-AF292559-1_at 1.756078333
AFFX-Dr-AF292559-2_s_at 3.911342895
AFFX-Dr-AF292559-3_s_at 2.745052186
AFFX-Dr-AF292559-4_s_at 1.833309849
AFFX-Dr-AF292560-1_s_at 2.710881995
AFFX-Dr-AF298789-1_at 1.884312024
AFFX-Dr-AF323980-1_at 2.295821184

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM322065.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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