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Sample GSM322066 Query DataSets for GSM322066
Status Public on Sep 18, 2008
Title Zebrafish_wt_24hpf, biological rep2
Sample type RNA
 
Source name Zebrafish embryo harvested at 24hpf
Organism Danio rerio
Characteristics Zebrafish wild-type whole-embryo RNA
Treatment protocol Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to manufacturer's (Affymetrix) recommendations
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description Gene expression data from wild-type embryos at 24 hours post fertilization
Data processing Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
 
Submission date Sep 17, 2008
Last update date Sep 17, 2008
Contact name Nathan Salomonis
E-mail(s) nathan.salomonis@cchmc.org
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1319
Series (1)
GSE12826 Zebrafish 24hpf mutant sk8 gene expression

Data table header descriptions
ID_REF
VALUE GC-RMA calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.673138989
AFFX-BioB-5_at 6.216712597
AFFX-BioB-M_at 6.844491206
AFFX-BioC-3_at 7.908094346
AFFX-BioC-5_at 7.941847012
AFFX-BioDn-3_at 10.07134914
AFFX-BioDn-5_at 9.478198211
AFFX-CreX-3_at 12.69532642
AFFX-CreX-5_at 12.0325046
AFFX-DapX-3_at 7.523969918
AFFX-DapX-5_at 6.230566259
AFFX-DapX-M_at 6.943554283
AFFX-Dr-AB076373-1_at 1.987037959
AFFX-Dr-AF292559-1_at 1.769029567
AFFX-Dr-AF292559-2_s_at 4.065711197
AFFX-Dr-AF292559-3_s_at 2.806207014
AFFX-Dr-AF292559-4_s_at 1.84617367
AFFX-Dr-AF292560-1_s_at 2.735963442
AFFX-Dr-AF298789-1_at 1.902715275
AFFX-Dr-AF323980-1_at 2.300713202

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM322066.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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