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Sample GSM322068 Query DataSets for GSM322068
Status Public on Sep 18, 2008
Title Zebrafish_mutant_sk8_24hpf, biological rep1
Sample type RNA
Source name Zebrafish embryo harvested at 24hpf
Organism Danio rerio
Characteristics Zebrafish mutant whole-embryo RNA
Treatment protocol Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to manufacturer's (Affymetrix) recommendations
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description Gene expression data from mutant embryos at 24 hours post fertilization
Data processing Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
Submission date Sep 17, 2008
Last update date Sep 17, 2008
Contact name Nathan Salomonis
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL1319
Series (1)
GSE12826 Zebrafish 24hpf mutant sk8 gene expression

Data table header descriptions
VALUE GC-RMA calculated Signal intensity

Data table
AFFX-BioB-3_at 5.518359127
AFFX-BioB-5_at 6.162897609
AFFX-BioB-M_at 7.332148588
AFFX-BioC-3_at 7.930495883
AFFX-BioC-5_at 7.922773759
AFFX-BioDn-3_at 10.30162104
AFFX-BioDn-5_at 9.613385017
AFFX-CreX-3_at 12.94128896
AFFX-CreX-5_at 12.27706969
AFFX-DapX-3_at 7.715028313
AFFX-DapX-5_at 5.996233066
AFFX-DapX-M_at 6.875041471
AFFX-Dr-AB076373-1_at 2.062271654
AFFX-Dr-AF292559-1_at 1.831730315
AFFX-Dr-AF292559-2_s_at 4.223264118
AFFX-Dr-AF292559-3_s_at 2.918400317
AFFX-Dr-AF292559-4_s_at 1.919778988
AFFX-Dr-AF292560-1_s_at 2.803338971
AFFX-Dr-AF298789-1_at 1.99014832
AFFX-Dr-AF323980-1_at 2.370202682

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.

Supplementary file Size Download File type/resource
GSM322068.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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