NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM322069 Query DataSets for GSM322069
Status Public on Sep 18, 2008
Title Zebrafish_mutant_sk8_24hpf, biological rep2
Sample type RNA
 
Source name Zebrafish embryo harvested at 24hpf
Organism Danio rerio
Characteristics Zebrafish mutant whole-embryo RNA
Treatment protocol Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to manufacturer's (Affymetrix) recommendations
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description Gene expression data from mutant embryos at 24 hours post fertilization
Data processing Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
 
Submission date Sep 17, 2008
Last update date Sep 17, 2008
Contact name Nathan Salomonis
E-mail(s) nathan.salomonis@cchmc.org
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1319
Series (1)
GSE12826 Zebrafish 24hpf mutant sk8 gene expression

Data table header descriptions
ID_REF
VALUE GC-RMA calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.736172747
AFFX-BioB-5_at 6.547566963
AFFX-BioB-M_at 7.10044005
AFFX-BioC-3_at 7.874269013
AFFX-BioC-5_at 7.978890857
AFFX-BioDn-3_at 10.15269567
AFFX-BioDn-5_at 9.502750671
AFFX-CreX-3_at 12.73743402
AFFX-CreX-5_at 12.24696987
AFFX-DapX-3_at 7.657700615
AFFX-DapX-5_at 6.582576588
AFFX-DapX-M_at 7.039345161
AFFX-Dr-AB076373-1_at 2.040332919
AFFX-Dr-AF292559-1_at 1.815658244
AFFX-Dr-AF292559-2_s_at 4.317399769
AFFX-Dr-AF292559-3_s_at 2.966249945
AFFX-Dr-AF292559-4_s_at 1.892977453
AFFX-Dr-AF292560-1_s_at 2.763286675
AFFX-Dr-AF298789-1_at 1.961239752
AFFX-Dr-AF323980-1_at 2.359528816

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM322069.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap